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By Rodolfo Paoletti, Dr. David Kritchevsky

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The fluorescent antibody technique is dif­ ficult to quantify, but Haust (1968) found albumin in small amounts in the superficial layers only in normal intima, whereas in early lesions it permeated into the deep layers; she was unable to demonstrate fibrin in normal intima, but it was present in all the fatty streaks examined, and in "white, fibrous plaques" where large amounts were demonstrated in the atheromatous "core" which showed only small, scattered deposits with the PTAH stain (Haust et al, 1964).

Reduced levels of GAG, which all investi­ gators find in advanced fibrous plaques (Smith, 1974) might allow more rapid penetration of lipoprotein into the deep layers of the intima. V. Relationship Between LD-Lipoprotein and the Lipid "Deposited" in Intima A. COMPARISON OF LIPOPROTEIN AND INTIMAL LIPIDS 1. Extracellular Lipid in Normal Intima In normal intima of young children, phospholipid is the major com­ ponent and most cholesterol is in the free (unesterified) form; the pre­ dominant fatty acids in the small cholesterol ester fraction are palmitic and oleic.

Compared with plasma there is a 7-fold decrease in albumin relative to lipoprotein in normal intima, and a 14-fold decrease 34 E L S P E T H B. SMITH Table XII PLASMA CONSTITUENTS IN INTIMA" Ratios in plasma and intima Concentration in plasma (mg/100 ml) Plasma Normal Gelatinous 4000 300 300 14 1 1 2 1 H 1 1 J4 Albumin LD-lipoprotein Fibrinogen Intima « From Smith (1974). in gelatinous lesions; fibrinogen shows a 3-fold decrease in normal intima, but is only half the plasma ratio in gelatinous lesions.

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